Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 109627
Title Bioefficacy of beta-carotene dissolved in oil studied in children in Indonesia
Author(s) Lieshout, M. van; West, C.E.; Muhilal, ; Permaesih, D.; Wang, Y.; Xu, X.; Breemen, R.B. van
Source American Journal of Clinical Nutrition 73 (2001). - ISSN 0002-9165 - p. 949 - 958.
DOI https://doi.org/10.1093/ajcn/73.5.949
Department(s) Human Nutrition & Health
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract More information on the bioefficacy of carotenoids in foods ingested by humans is needed. Objective: We aimed to measure the time required for isotopic enrichment of β-carotene and retinol in serum to reach a plateau, the extent of conversion of β-carotene dissolved in oil with use of β-carotene and retinol specifically labeled with 10 13C atoms, and the intraindividual variation in response. Design: Indonesian children aged 8-11 y (n = 35) consumed 2 capsules/d, 7 d/wk, for ≤ 10 wk. Each capsule contained 80 μg [12,13,14,15,20,12′,13′,14′,15′,20′- 13C10]β-carotene and 80 μg [8,9,10,11,12,13,14,15,19,20-13C10] retinyl palmitate. Three blood samples were drawn per child over a period of ≤ 10 wk. HPLC coupled with atmospheric pressure chemical ionization liquid chromatography-mass spectrometry was used to measure the isotopic enrichment in serum of retinol with [13C5]retinol and [13C10]retinol and of β-carotene with [13C10] β-carotene. The β-carotene in the capsules used had a cis-trans ratio of 3:1. Results: Plateau isotopic enrichment was reached by day 21. The amount of β-carotene in oil required to form 1 μg retinol was 2.4 μg (95␌I: 2.1, 2.7). The amount of all-trans-β-carotene required to form 1 μg retinol may be lower. Conclusions: The efficiency of conversion of this β-carotene in oil was 27␋etter than that estimated previously (1.0 μg retinol from 3.3 μg β-carotene with an unknown cis-trans ratio). The method described can be extended to measure the bioefficacy of carotenoids in foods with high precision, requiring fewer subjects than other methods.
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