Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 109731
Title Ig light chain variability in DNP494-KLH immunised sea bass (Dicentrarchus labrax L.) : evidence for intra-molecular induced suppression
Author(s) Santos, N.M.S. dos; Hermsen, T.; Rombout, J.H.W.M.; Pilström, L.; Stet, R.J.M.
Source Developmental and Comparative Immunology 25 (2001). - ISSN 0145-305X - p. 387 - 401.
DOI https://doi.org/10.1016/S0145-305X(01)00002-7
Department(s) Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract The coding sequence of the sea bass light chain was obtained by sequential anchored PCR on a head kidney cDNA library of a DNP494-KLH immunised sea bass. The cDNA sequence obtained codes for a leader peptide of 21 aa and a mature IgL chain of 223 aa. Both the amino acid sequence comparisons and neighbour-joining trees showed that the IgL chain of sea bass obtained is of the L1/G type. To study the variability of the light chain, additional PCRs on the cDNA library and cDNA from pooled head kidneys were performed. Multiple alignment of unique sequences (N=17) could be performed without introducing gaps, and showed extremely low variability in CDR1, and no variability in CDR2 or CDR3. A possible explanation for this low variability of the IgL1 chain might be the enhanced expression of monospecific anti-DNP antibodies. The isolation and characterisation of partial genomic and cDNA IgL sequences, which showed normal variability, corroborate this explanation
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