Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 109900
Title Progress made in FEC transformation of cassava
Author(s) Raemakers, K.; Schreuder, M.; Pereira, I.; Munyikwa, T.; Jacobsen, E.; Visser, R.
Source Euphytica (2001)Special issue. - ISSN 0014-2336 - p. 15 - 24.
Department(s) Plant Breeding
Publication type Non-refereed article in scientific journal
Publication year 2001
Abstract In cassava friable embryogenic callus (FEC) has been used to obtain transgenic plants using particle bombardment, electroporation, and Agrobacterium tumefaciens. FEC cultures have been obtained in 6 of the 10 tested genotypes. In all genotypes FEC could be regenerated into plants, however the efficiency differed between the genotypes. Almost all plants regenerated from 6 months old FEC cultures of TMS604444, Adira 4, Thai 5 and M7 were morphological similar to control plants. However, in R60 and R90 a large number of plants were not identical to control plants. Older FEC lines of TMS60444 have a reduced ability to regenerate plants and the plants show somaclonal variation. Somaclonal variation is observed in the same extend in transgenic and non-transgenic plants. The origin of this variation is both genetic and epigenetic. Luciferase based selection is less efficient in producing transgenic lines than chemical selection. Furthermore Agrobacterium tumefaciens mediated transformation is much more efficient than particle bombardment with respect to the production of transgenic lines. A tentative model is introduced which best describes the effect of different selection regimes on the time period required to produce transgenic plants. Kanamycin and stringent luciferase selection required a shorter period of time than selection based on hygromycin, phosphinothricin or non-stringent luciferase. However, a more significant reduction of time was obtained if young instead of old FEC lines of genotype TMS60444 were used for genetic modification. In accordance to the model these young FEC lines of TMS60444 produced transgenic plants within 4 months with both Agrobacterium tumefaciens combined with kanamycin selection and particle bombardment combined with stringent luciferase selection.
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