Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 110199
Title Estimation of steady-state culture characteristics during acceleration-stats with yeasts
Author(s) Sluis, C. van der; Westerink, B.H.; Dijkstal, M.M.; Castelein, S.J.; Boxtel, A.J.B. van; Tramper, J.; Wijffels, R.H.
Source Biotechnology and Bioengineering 75 (2001)3. - ISSN 0006-3592 - p. 267 - 275.
Department(s) Sub-department of Food and Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract Steady-state culture characteristics are usually determined in chemostat cultivations, which are very time-consuming. In contrast, acceleration-stat (A-stat) cultivations in which the dilution rate is continuously changed with a constant acceleration rate are not so time-consuming, especially at high acceleration rates. Therefore, the A-stat could be advantageous to use instead of the chemostat. However, the highest acceleration rate, meaning the fastest A-stat that can be applied for estimating steady-state culture characteristics, is not known yet. Experimental results obtained with Zygosaccharomyces rouxii, an important yeast in soy sauce processes, showed that the culture characteristics during the A-stat with an acceleration rate of 0.001 h-2 were roughly comparable to those of the chemostat. For higher acceleration rates the deviation between the culture characteristics in the A-stat and those in the chemostat obtained at the same dilution rate generally started to increase. The source of these deviations was examined by simulation for Saccharomyces cerevisiae. The simulations demonstrated that this deviation was not only dependent on the metabolic adaptation rate of the yeast, but also on the rate of change in environmental substrate concentrations during A-stats. From this work, it was concluded that an A-stat with an acceleration rate of 0.001 h-2 is attractive to be used instead of chemostat whenever a rough estimation of steady-state culture characteristics is acceptable
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