Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 110259
Title The effect of oxygen on methanol oxidation by an obligate metahnotrophic bacterium studied by in vivo 13C nuclear magnetic resonance spectroscopy
Author(s) Costa, C.; Vecherskaya, M.; Dijkema, C.; Stams, A.J.M.
Source Journal of Industrial Microbiology and Biotechnology 26 (2001). - ISSN 1367-5435 - p. 9 - 14.
DOI https://doi.org/10.1038/sj.jim.7000075
Department(s) Microbiology
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract 13C NMR was used to study the effect of oxygen on methanol oxidation by a type II methanotrophic bacterium isolated from a bioreactor in which methane was used as electron donor for denitrification. Under high (35-25€oxygen conditions the first step of methanol oxidation to formaldehyde was much faster than the following conversions to formate and carbon dioxide. Due to this the accumulation of formaldehyde led to a poisoning of the cells. A more balanced conversion of 13C-labelled methanol to carbon dioxide was observed at low (1-5€oxygen concentrations. In this case, formaldehyde was slowly converted to formate and carbon dioxide. Formaldehyde did not accumulate to inhibitory levels. The oxygen-dependent formation of formaldehyde and formate from methanol is discussed kinetically and thermodynamically.
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