Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 110410
Title Analysis of taxines in Taxus plant material and cell cultures by hplc photodiode array and hplc-electrospray mass spectrometry
Author(s) Theodoridis, G.; Laskaris, G.; Rozendaal, E.L.M.; Verpoorte, R.
Source Journal of liquid chromatography & related technologies 24 (2001)15. - ISSN 1082-6076 - p. 2267 - 2282.
Department(s) Organic Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract A semi-purified Taxus baccata needles extract was analysed by RP-HPLC. More than 18 taxines and cinnamates were detected by photodiode array detection and LC-MS, 10 of them being positively identified. Furthermore, 10-deacetyl baccatin III (paclitaxel's main precursor) and other taxanes were also found in the extract. Taxines were also detected in numerous extracts of plant and cell culture extracts of various origins. The identification of the taxines in the extracts was made by correlation of retention and spectral data. LC-electrospray MS verified the identification of the known taxines in T. baccata seeds, needles, and pollen
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