|Title||Analysis of post-harvest deterioration in tuberous roots of cassava (Manihot esculenta Grantz)|
|Source||Wageningen University. Promotor(en): R.G.F. Visser; E. Jacobsen. - S.l. : S.n. - ISBN 9789058085481 - 103|
|Publication type||Dissertation, internally prepared|
|Keyword(s)||manihot esculenta - cassave - bederf na de oogst - bewaarfysiologie - biochemie - enzymen - genetische merkers - genetische transformatie - moleculaire genetica - transgene planten - manihot esculenta - cassava - postharvest decay - postharvest physiology - biochemistry - enzymes - genetic markers - genetic transformation - molecular genetics - transgenic plants|
This thesis deals with the analysis of postharvest physiological deterioration (PPD) in cassava tuberous roots at the physiological, biochemical and molecular level. By setting up a uniform visual system to monitor the onset and maintenance of PPD under the experimental conditions, amongst others physico-chemical properties of starch were characterized. Furthermore using this system, around 6,000 TDFs (transcript derived fragments) were screened via 100 primer combinations using cDNA-AFLP. 70 TDFs showing an up-regulated, a down regulated or a transiently expression pattern were isolated following the first 72 hours of PPD. Based on the sequence information, a functional catalogue of these TDFs was established. By concentrating on enzymes possibly involved in oxidative stress, biochemical results indicated that PPD may be a peroxidase-mediated process. Using a reverse genetics approach, the putative cassava dad1 (defender against cell death gene 1) homologue was transformed into cassava FEC (friable embryogenic callus) lines. Transgenic plants were produced and characterized.