Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 110610
Title Secretory pathway limits the enhanced expression of classical swine fever virus E2 glycoprotein in insect cells
Author(s) Oers, M.M. van; Thomas, A.A.M.; Moormann, R.J.M.; Vlak, J.M.
Source Journal of Biotechnology 86 (2001). - ISSN 0168-1656 - p. 31 - 38.
DOI https://doi.org/10.1016/S0168-1656(00)00403-X
Department(s) ID Lelystad, Institute for Animal Science and Health
Laboratory of Virology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract The 3′ untranslated region (UTR) is an important element that determines the level of recombinant protein expression via baculovirus vectors. Previous work using chloramphenicol acetyl transferase as reporter has shown that p10-promoter based baculovirus vectors with the authentic p10 3′ UTR resulted in higher expression levels than vectors carrying an SV40 early terminator, as part of a lacZ selection cassette. To examine whether a similar increase in expression levels could be obtained for baculovirus-expressed glycoproteins, the classical swine fever virus E2 antigen was used as a model. With the authentic p10 3′ UTR higher levels of E2 transcript were found than in the presence of the SV40 terminator. This higher number of transcripts was accompanied by elevated levels of intracellular, non-glycosylated E2 protein. However, the levels of intracellular glycosylated forms of E2 and of extracellular E2 were similar for both type of terminators. These results show that translation of the recombinant mRNA is not the rate limiting step in the expression of glycoproteins, but the downstream processing and secretion of the translation products.
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