Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 110712
Title No evidence for binding between resistance gene product Cf-9 of tomato and avirulence gene product AVR9 of Cladosporium fulvum
Author(s) Luderer, R.; Rivas, S.; Nurnberger, T.; Mattei, B.; Hooven, H.W. Van den; Hoorn, R.A.L. Van der; Romeis, T.; Wehrfritz, J.M.; Blume, B.; Nennstiel, D.; Zuidema, D.; Vervoort, J.J.M.; Lorenzo, G. De; Jones, J.D.G.; Wit, P.J.G.M. De; Joosten, M.H.A.J.
Source Molecular Plant-Microbe Interactions 14 (2001)7. - ISSN 0894-0282 - p. 867 - 876.
DOI https://doi.org/10.1094/MPMI.2001.14.7.867
Department(s) Biochemistry
Laboratory of Phytopathology
Laboratory of Virology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract The gene-for-gene model postulates that for every gene determining resistance in the host plant, there is a corresponding gene conditioning avirulence in the pathogen. On the basis of this relationship, products of resistance (R) genes and matching avirulence (Avr) genes are predicted to interact. Here, we report on binding studies between the R gene product Cf-9 of tomato and the Avr gene product AVR9 of the pathogenic fungus Cladosporium fulvum. Because a high-affinity binding site (HABS) for AVR9 is present in tomato lines, with or without the Cf-9 resistance gene, as well as in other solanaceous plants, the Cf-9 protein was produced in COS and insect cells in order to perform binding studies in the absence of the HABS. Binding studies with radio-labeled AVR9 were performed with Cf-9-producing COS and insect cells and with membrane preparations of such cells. Furthermore, the Cf-9 gene was introduced in tobacco, which is known to be able to produce a functional Cf-9 protein. Binding of AVR9 to Cf-9 protein produced in tobacco was studied employing surface plasmon resonance and surface-enhanced laser desorption and ionization. Specific binding between Cf-9 and AVR9 was not detected with any of the procedures. The implications of this observation are discussed.
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