Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 110743
Title Metabolic response of roots to osmotic stress in sensitive and tolerant cereals - Qualitative in vivo [P-31] nuclear magnetic resonance study
Author(s) Nagarajan, S.; Dijkema, C.; As, H. van
Source Indian journal of biochemistry and biophysics 38 (2001). - ISSN 0301-1208 - p. 149 - 152.
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract High resolution [31P] nuclear magnetic resonance (NMR) spectroscopy was used to investigate the changes in phosphate metabolism and intracellular pH in intact root segments of relatively osmotic stress sensitive species maize (Zea mays L) and insensitive species pearl millet (Pennisetum americanum (L) Leeke) exposed to hyper osmotic shock. The results were used to understand the adaptive mechanism of the two species. The hyper osmotic shock resulted in large build-up of phosphocholine and decrease in glucose 6-phosphate (G-6P) and UDPG levels in both the crops. The osmotic shock produced a large vacuolar alkalinization and decrease in pH across tonoplast membrane in maize roots. However, the roots of pearl millet were able to adapt to the stress and maintained pH gradient across tonoplast with marginal vacuolar alkalinization. This may be attributed to the sustained activity of primary tonoplast pumps and increased activity of H -ATPaSe that normally maintain pH gradient across tonoplast.
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