Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 121375
Title Characterisation of a monoclonal antibody to carp IL-1 beta and the development of a sensitive capture ELISA
Author(s) Mathew, J.A.; Guo, Y.X.; Goh, K.P.; Chan, J.; Verburg-van Kemenade, B.M.L.; Kwang, J.
Source Fish and Shellfish Immunology 2 (2002). - ISSN 1050-4648 - p. 85 - 95.
DOI https://doi.org/10.1006/fsim.2001.0383
Department(s) Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract A carp IL-1 gene was identified from a subtraction hybridisation technology based cDNA library from activated carp leucocytes. This gene was cloned into pQE vector carrying 6xHis tag and the protein was expressed. Recombinant IL-1 was used to produce hybridomas specific for carp IL-1. Monoclonal antibodies were purified by affinity column and a sandwich ELISA for IL-1 was developed with a detection limit of 10 ng of the recombinant protein. Using the capture ELISA, the presence of native IL-1 in culture supernatant of PHA-stimulated leucocytes from carp was identified, which was confirmed by SDS-PAGE and Western blot. Since IL-1 is known to stimulate proliferation of T & B cells and macrophages, its ability to stimulate proliferation of carp leucocytes was studied using tritiated thymidine. The recombinant protein was found to significantly stimulate proliferation of head kidney and spleen cells from carp
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