ID Lelystad, Institute for Animal Science and Health
The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 X 10<5> to 5 X 10<6> cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E<rns> protein or fragments thereof in insect cell culture characterised by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 mu g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, alpha -units and/or beta -units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 mu /ml.
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