Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 305761
Title Protein expression in baculovirus vector expression systems
Author(s) Moormann, R.J.M.; Kretzdorn, D.; Hamann, E.K.; Smit, A.J. de
Source Octrooinummer: WO9931257, verleend: 1997-11-01.
Department(s) ID Lelystad, Institute for Animal Science and Health
Publication type Patent
Publication year 1997
Abstract The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 X 10<5> to 5 X 10<6> cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E<rns> protein or fragments thereof in insect cell culture characterised by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 mu g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, alpha -units and/or beta -units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 mu /ml.
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