Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 318378
Title Enzymatic synthesis of thioglucosides using almond ß-glucosidase
Author(s) Meulenbeld, G.H.; Roode, B.M. de; Hartmans, S.
Source Biocatalysis and Biotransformation 20 (2002)4. - ISSN 1024-2422 - p. 251 - 256.
Department(s) Organic Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract A selection of different glycosidases was screened for the glycosylation of 1-propanethiol. The g-glucosidases from almond, Aspergillus niger and Caldocellum saccharolyticum were capable of 1-propanethioglucoside (1-PTG) formation. The almond g-glucosidase showed the highest activity in this reversed hydrolysis type of reaction using glucose as glucosyl donor. Besides 1-propanethiol, also thioglucosides of 2-propanethiol and furfuryl mercaptan were formed by the almond g-glucosidase. The substrate specificity of the almond g-glucosidase with respect to thioglucosylation is restricted to primary and secondary aliphatic thiols. Once the thioglucosides are formed, they are not hydrolyzed at a significant rate by almond g-glucosidase. As a consequence the synthesis of 1-PTG could be observed at very low aglycone concentrations (0.5␟/v based on the reaction solution) and high yields (68␋ased on 1-PT and 41␋ased on glucose) were obtained. An excess of aglycone, otherwise frequently applied in reversed hydrolysis glycosylation, is therefore not necessary in the glucosylation of 1-PT.
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