Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 318733
Title Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases
Author(s) Lücker, J.; Tamer, M.K. El; Schwab, W.; Verstappen, F.W.A.; Plas, L.H.W. van der; Bouwmeester, H.J.; Verhoeven, H.A.
Source European Journal of Biochemistry 269 (2002). - ISSN 0014-2956 - p. 3160 - 3161.
Department(s) Laboratory of Plant Physiology
Plant Research International
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract Citrus limon possesses a high content and large variety of monoterpenoids, especially in the glands of the fruit flavedo. The genes responsible for the production of these monoterpenes have never been isolated. By applying a random sequencing approach to a cDNA library from mRNA isolated from the peel of young developing fruit, four monoterpene synthase cDNAs were isolated that appear to be new members of the previously reported tpsb family. Based on sequence homology and phylogenetic analysis, these sequences cluster in two separate groups. All four cDNAs could be functionally expressed in Escherichia coli after removal of their plastid targeting signals. The main products of the enzymes in assays with geranyl diphosphate as substrate were ( )-limonene (two cDNAs) (-)--pinene and -terpinene. All enzymes exhibited a pH optimum around 7; addition of Mn2 as bivalent metal ion cofactor resulted in higher activity than Mg2 , with an optimum concentration of 0.6 mm. Km values ranged from 0.7 to 3.1 ?m. The four enzymes account for the production of 10 out of the 17 monoterpene skeletons commonly observed in lemon peel oil, corresponding to more than 90␘f the main components present
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