Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 319908
Title A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties
Author(s) Roldán-Ruiz, I.; Eeuwijk, F.A. van; Gilliland, T.J.; Dubreuil, P.; Dillmann, C.; Lallemand, J.; Loose, M. De; Baril, C.P.
Source Theoretical and Applied Genetics 103 (2001)8. - ISSN 0040-5752 - p. 1138 - 1150.
DOI https://doi.org/10.1007/s001220100571
Department(s) Plant Breeding
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2001
Keyword(s) european barley germplasm - genetic diversity - species relationships - aflp markers - dna - cultivars - rapd - rflps - pedigree - plant
Abstract A sample set of registered perennial ryegrass varieties was used to compare how morphological characterisation and AFLP® (AFLP® is a registered trademark of Keygene N.V.) and STS molecular markers described variety relationships. All the varieties were confirmed as morphologically distinct, and both the STS and AFLP markers exposed sufficient genetic diversity to differentiate these registered ryegrass varieties. Distances obtained by each of the approaches were compared, with special attention given to the coincidences and divergences between the methods. When correlations between morphological, AFLP and STS distances were calculated and the corresponding scatter-plots constructed, the variety relationships appeared to be rather inconsistent across the methods, especially between morphology and the molecular markers. However, some consistencies were found for closely related material. An implication could be that these molecular-marker techniques, while not yet suited to certain operations in the traditional registration of new varieties, could be suitable methods for investigating disputable distinctness situations or possible EDV (EDV= essentially derived variety. An EDV is a variety being clearly distinct from, but conforming in the expression of the essential characteristics of, an ¿initial variety' (IV) from which it is found to have been predominantly derived) relationships, subject to establishing standardised protocols and statistical techniques. Some suggestions for such a protocol, including a statistical test for distinctness, are given
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