Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320160
Title Effects of oxytocin on semen release response in African catfish (Clarias gariepinus)
Author(s) Viveiros, A.T.M.; Jatzkowski, A.; Komen, J.
Source Theriogenology 59 (2003)9. - ISSN 0093-691X - p. 1905 - 1917.
DOI https://doi.org/10.1016/S0093-691X(02)01290-6
Department(s) Aquaculture and Fisheries
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) epididymis - quality - spermatogenesis - localization - vasopressin - receptors - hormones
Abstract In silurid fishes, semen collection is practically impossible, even after hormonal stimulation. Instead, males are killed and testes macerated to obtain sperm. To understand the endocrine control of semen release in catfishes, we investigated the role of smooth muscle contractors in semen release and semen quality of African catfish (Clarias gariepinus). In in vitro experiments, testis slices were incubated with oxytocin (1 and 10 IU), isotocin (2 and 20 ug), vasopressin (0.2 and 2 ug), epinephrine (1 and 10 ug), PGF2alpha (1 and 10 ug), purified Clarias LH (300 ng) and partly purified Clarias pituitary extract (containing 300 ng LH). Only oxytocin increased sperm concentration of the medium (assessed by optical density measurements) compared to control incubations. Oxytocin was then tested in vivo in two groups of fish: normal males, and males that had been treated with 17alpha-methyltestosterone during larval stages to inhibit seminal vesicle development (NIT males). Both groups of fish received two doses of carp pituitary suspension (8 and 10 mg/kg, respectively i.m.) with or without subsequent oxytocin treatment (5 IU/kg i.v.; cPS-OT treatment and cPS treatment, respectively). There was no effect of oxytocin on the number of strippable males. Of cPS and cPS-OT treated fish, 87% MT males and 60% normal males were, strippable. The stripped semen volume was low in both groups but MT males produced higher (P <0.001) hatching rates (63.1%) than did normal males (2.1%). (C) 2002 Elsevier Science Inc. All rights reserved.
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