Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320170
Title Ultrafast polarized fluorescence measurements on Tryptophan and a Tryptophan-containing peptide
Author(s) Larsen, O.F.A.; Stokkum, I.H.M. van; Pandit, A.; Grondelle, R. van; Amerongen, H. van
Source The Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical 107 (2003). - ISSN 1520-6106 - p. 3080 - 3085.
DOI https://doi.org/10.1021/jp021756i
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) conformational dynamics - excited-state - alpha-helix - hiv-1 rev - proteins - indole - decay - rna - depolarization - spectroscopy
Abstract In this work polarized picosecond fluorescence measurements were performed on isolated tryptophan and tryptophan in a small 22-mer peptide using a streak camera coupled to a spectrograph as a detection system. In both cases the fluorescence decay was multiexponential with decay times of similar to500 ps, and similar to4 ns. Surprisingly, also a short-lived (similar to16 ps) isotropic fluorescence component was found for both tryptophan and the peptide which, to our knowledge. has never been observed before. Fluorescence anisotropy data showed rotational correlation times of 155 ps and 1.5 ns for the peptide, reflecting local and overall peptide dynamics, respectively. Recently it was argued [Lakowicz. J. R. Photochem. Photobiol. 2000, 72, 421] that the nonexponential fluorescence decay of tryptophan in proteins is mainly due to spectral relaxation, whereas for isolated tryptophan it is due to different rotamers. Our results do not support this view. In contrast we conclude in both cases that the different fluorescence decay times should be ascribed to different rotameric states.
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