Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320273
Title Molecular and functional characterization of carp TNF: a link between TNF polymorphism and trypanotolerance?
Author(s) Saeij, J.P.J.; Stet, R.J.M.; Vries, B.J. de; Muiswinkel, W.B. van; Wiegertjes, G.F.
Source Developmental and Comparative Immunology 27 (2003)1. - ISSN 0145-305X - p. 29 - 41.
DOI https://doi.org/10.1016/S0145-305X(02)00064-2
Department(s) Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) tumor-necrosis-factor - alpha messenger-rna - common carp - cyprinus-carpio - nitric-oxide - 3'-untranslated region - complement c3 - expression - cloning - pentoxifylline
Abstract Two carp tumor necrosis factor alpha (TNF¿) genes have been cloned and sequenced. Both TNF1 and TNF2 sequences have several polymorphisms in the 3¿ UTR and TNF2 has a polymorphism in the coding sequence. Lipopolysaccharide and the protozoan blood flagellate Trypanoplasma borreli induced expression of TNF¿ in carp head kidney phagocytes when added in vitro. Differential expression was observed, with TNF2 being higher expressed than TNF1. We used the TNF¿-specific inhibitor pentoxifylline to demonstrate the involvement of carp TNF¿ in the induction of nitric oxide and in the stimulation of cell proliferation. In addition, two carp lines differing in their resistance to T. borreli were typed for the TNF2 polymorphism and association between one isoform and resistance was found.
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