Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320285
Title The cytoskeleton and the secretory pathway are not involved in targeting the cowpea mosaic virus movement protein to the cell periphery
Author(s) Pouwels, J.; Krogt, G.N.M. van der; Lent, J. van; Bisseling, T.; Wellink, J.E.
Source Virology 297 (2002). - ISSN 0042-6822 - p. 48 - 56.
DOI https://doi.org/10.1006/viro.2002.1424
Department(s) Laboratory of Molecular Biology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2002
Keyword(s) green fluorescent protein - endoplasmic-reticulum - tubular structures - m-rna - intracellular-distribution - plasma-membrane - plant - plasmodesmata - protoplasts - microtubules
Abstract The movement protein (MP) of cowpea mosaic virus (CPMV) forms tubules on infected protoplasts and through plasmodesmata in infected plants. In protoplasts the MP fused to GFP (MP-GFP) was shown to localize in peripheral punctate structures and in long tubular structures extending from the protoplast surface Using cytoskeletal assembly inhibitors (latrunculin B and oryzalin) and an inhibitor of the secretory pathway (brefeldin A), targeting of the MP to the peripheral punctate structures was demonstrated not to be dependent on an intact cytoskeleton or functional secretion pathway, Furthermore it was shown that a disrupted cytoskeleton had no effect on tubule formation but that the addition of brefeldin A, severely inhibited tubule formation. The results presented in this paper suggest a role for a plasma membrane host factor in tubule formation of plant viral MPs, (C) 2002 Elsevier Science (USA).
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