Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320399
Title Early in vivo cytokine genes expression in chickens after challenge with Salmonella typhimurium lipopolysaccharide and modulation by dietary n-3 polyunsaturated fatty acids
Author(s) Sijben, J.W.C.; Klasing, K.C.; Schrama, J.W.; Parmentier, H.K.; Poel, J.J. van der; Savelkoul, H.F.J.; Kaiser, P.
Source Developmental and Comparative Immunology 27 (2003). - ISSN 0145-305X - p. 611 - 619.
Department(s) Adaptation Physiology
Aquaculture and Fisheries
Animal Breeding and Genomics
Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) growing layer hens - fish-oil - interferon-gamma - functional-characterization - turkey interferon - ifn-gamma - t-cells - growth - il-15 - blood
Abstract We studied the effects of Salmonella typhimurium lipopolysaccharide (LPS) on in vivo cytokine mRNA levels in chickens, and investigated whether these levels could be altered by different nutrients. Two hundred and forty chicks were assigned in a 2 X 4 factorial design of treatments. Factors were intravenous injection with S. typhimuriam LPS, or saline (control), and four dietary fat sources: corn oil (CO), linseed oil (LO), menhaden oil and beef tallow (BT). Two hours after injection birds were killed and their spleens removed for RNA extraction. Quantitative real-time RT-PCR assays for mRNA of chicken IFN-gamma, IL-6, IL-8, IL-15, IL-18 and 28S rRNA were used to obtain the in vivo splenic cytokine profiles. Expression levels of IL-6, IL-8, IL-18 and IFN-gamma mRNA increased, but IL-15 mRNA decreased 2 h after challenge with LPS compared with saline controls. In saline-injected control chickens, the dietary oil source did not affect the splenic mRNA level of any cytokine. In LPS challenged chickens IFN-gamma mRNA was significantly higher in the chickens fed the fish oil enriched diet compared with the LO, CO and BT enriched diets. The present data imply that avian IL-15 has, at least partially, a different function compared to its mammalian counterpart, and in addition, chicken innate immune responses might be affected differently by n - 3 PUFA compared to mammals. (C) 2003 Published by Elsevier Science Ltd.
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