Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320410
Title A new and versatile method for the successful conversion of AFLP-TM markers into simple single locus markers
Author(s) Brugmans, B.W.; Hulst, R.G.M. van der; Visser, R.G.F.; Lindhout, W.H.; Eck, H. van
Source Nucleic acids research 31 (2003)10. - ISSN 0305-1048 - p. e55 - e55.
DOI https://doi.org/10.1093/nar/gng055
Department(s) Plant Breeding
EPS-3
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) fragment-length-polymorphism - pcr-based markers - suppressed recombination - f-2 populations - rflp markers - dna - resistance - barley - gene - map
Abstract Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus-specific PCR primers. In addition, single locus polymorphism (SNP) information is required to design an allele-specific assay. This paper describes a new and versatile method for the conversion of AFLP markers into simple assays. The protocol presented in this paper offers solutions for frequently occurring pitfalls and describes a procedure for the identification of the SNP responsible for the AFLP. By following this approach, a high success rate for the conversion of AFLP markers into locus-specific markers was obtained.
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