Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 321439
Title Selective interaction between Xanthophylls and Chlorophylls in LHCII probed by femtosecond transient absoprtion spectroscopy
Author(s) Gradinaru, C.C.; Grondelle, R. van; Amerongen, H. van
Source The Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical 107 (2003). - ISSN 1520-6106 - p. 3938 - 3943.
DOI https://doi.org/10.1021/jp026278q
Department(s) Biophysics
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) light-harvesting complex - higher-plants - energy-transfer - photosystem-ii - green plants - antenna complexes - protein complex - binding-site - carotenoids - dynamics
Abstract We have performed femtosecond transient absorption measurements on trimeric light-harvesting complex II from spinach. Either chlorophyll (Chl) a (675 nm) or Chl b (650 nm) was excited, and the spectral response was probed for wavelengths longer than 470 nm. Excitation of Chl b led to instantaneous bleaching of two distinct Chl b bands with absorption peaks at 473 and 486 nm, corresponding to different Chl b subpopulations. The latter band probably also contains a contribution from a neoxanthin molecule, which is strongly coupled to Chl b. Most of the subsequent energy transfer from Chl b to Chl a occurs well within a picosecond, but energy transfer from the Chl b subpopulation with an absorption peak at 473 nm occurs faster (several hundreds of femtoseconds). Excitation of Chl b does not lead to a detectable instantaneous response of the lutein molecules with absorption peaks at 494 and 510 nm. This is in contrast with excitation of Chl a, which in turn does not lead to a detectable response of the neoxanthin or Chl b molecules. Excitation of Chl a leads to concomitant small bleachings of both lutein molecules at 494 and 510 nm (2% and 1% respectively of the Chl a bleaching) which we ascribe to excitonic mixing of the S, states of the lutein molecules and the Q(y) states of the Chl a molecules.
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