Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 322199
Title The presence of a chromatin boundary appears to shield a transgene in tobacco from RNA silencing
Author(s) Mlynarova, L.; Hricova, A.; Loonen, A.; Nap, J.P.H.
Source The Plant Cell 15 (2003). - ISSN 1040-4651 - p. 2203 - 2217.
Department(s) Laboratory of Molecular Biology
PRI Bioscience
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) matrix attachment regions - dna methylation - messenger-rna - domain boundaries - single-copy - gene - expression - plants - suppression - interference
Abstract We present isogenic transgenic tobacco lines that carry at a given chromosomal position a -glucuronidase (GUS) reporter gene either with or without the presence of the matrix-associated region known as the chicken lysozyme A element. Plants were generated with the Cre-lox site-specific recombination system using heterospecific lox sites. Analysis of GUS gene expression in plant populations demonstrates that the presence of the A element can shield against RNA silencing of the GUS gene. Protection was observed in two of three independent tobacco transformants. Plants carrying an A element 5' of the GUS gene always had stable GUS activity, but upon removal of this A element, the GUS gene became silenced over time in two lines, notably when homozygous
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