Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 325321
Title Spatio-temporal patterns of photosystem II activity and plasma-membrane proton flows in Chara corallina cells exposed to overall and local illumination
Author(s) Bulychev, A.A.; Vredenberg, W.J.
Source Planta 218 (2003). - ISSN 0032-0935 - p. 143 - 151.
DOI https://doi.org/10.1007/s00425-003-1084-6
Department(s) Laboratory of Plant Physiology
EPS-3
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) chlorophyll-a fluorescence - photosynthetic activity - internodal cells - basic zones - k+ channel - light - oscillations - transport - nitella - ph
Abstract Pulse-amplitude modulated microfluorometry and an extracellular pH microprobe were used to examine light-induced spatial heterogeneity of photosynthetic and H+-transporting activities in cells of Chara corallina Klein ex Willd. Subcellular domains featuring different PSII photochemical activities were found to conform to alternate alkaline and acid zones produced near the cell surface, with peaks of PSII activity correlating with the position of acid zones. Buffers eliminated pH variations near the cell surface but did not destroy the variations in PSII photochemical yield (DeltaF/F-m'). When a dark-adapted cell was exposed to actinic light, the PSII effective yield decreased within 5-15 min in the alkaline regions but rose after the initial decline in the acid regions. The light-induced decrease in DeltaF/F-m' in the alkaline regions occurred prior to or synchronously with the steep rise in local pH. The kinetics of DeltaF/F-m', F-m', and F observed in alkaline regions under overall illumination of Chara cells were replaced by those typical of acid regions, when the illumination area size was restricted to 1.5-2 mm. The data show that photoinduced patterns in photosynthetic activity are not predetermined by the particular structural organization of alkaline and acid cell regions but are subject to dynamic changes.
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