Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 325744
Title The life and death of sponge cells
Author(s) Sipkema, D.; Snijders, A.P.L.; Schroën, C.G.P.H.; Osinga, R.; Wijffels, R.H.
Source Biotechnology and Bioengineering 85 (2004)3. - ISSN 0006-3592 - p. 239 - 247.
Department(s) Bioprocess Engineering
Food Process Engineering
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) marine natural-products - in-vitro - growth-inhibition - primary culture - ammonium ion - heat-stress - trehalose - sea - identification - cytotoxicity
Abstract Cell viability is an essential touchstone in the study of the effect of medium components on cell physiology. We developed a flow-cytometric assay to determine sponge-cell viability, based on the combined use of fluorescein diacetate (FDA) and propidium iodide (PI). Cell fluorescence measurements based on incubation of cells with FDA or PI resulted in a useful and reproducible estimate of the viability of primary sponge-cell cultures. We studied the effects of temperature, ammonium, and the fungicide amphotericin B on the viability of a primary-cell culture from the marine sponge Suberites domuncula using the aforementioned flow-cytometric assay. S. domuncula cells die rapidly at a temperature of greater than or equal to22degreesC, but they are insensitive to ammonium concentrations of up to 25 mM. Amphotericin B, which is frequently used in sponge-cell culture media, was found to be toxic to S. domuncula cells. (C) 2004 Wiley Periodicals, Inc.
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