Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 325964
Title Mode of action of Fusarium moniliforme endopolygalacturonase towards acetylated pectin.
Author(s) Bonnin, E.; Alebeek, G.J.W.M. van; Voragen, A.G.J.; Thibault, J.F.
Source Carbohydrate Polymers 52 (2003). - ISSN 0144-8617 - p. 381 - 388.
DOI https://doi.org/10.1016/S0144-8617(02)00332-6
Department(s) Food Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) aspergillus-niger - endo-polygalacturonase - methylation - acid - degradation - hydrolysis
Abstract Endopolygalacturonase from Fusarium moniliforme was used to degrade acetylated homogalacturonan previously prepared from sugar beet pulp. The initial velocity and the final percentage of hydrolysis decreased-very rapidly with increasing degree of acetylation, showing that acetyl substitution markedly affected the enzymatic activity. MALDI-TOF mass spectrometry was used to analyse the reaction products and to show acetyl groups on the oligogalacturonates. The results demonstrated that the enzyme was able to accommodate acetyl groups in its active site cleft. The influence of acetyl groups on the mode of action of the enzyme was discussed and compared to the influence of methyl groups. (C) 2003 Elsevier Science Ltd. All rights reserved.
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