Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 325991
Title Negative -strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions
Author(s) Bucher, E.C.; Sijen, T.; Haan, P. de; Goldbach, R.W.; Prins, M.W.
Source Journal of Virology 77 (2003). - ISSN 0022-538X - p. 1329 - 1336.
DOI https://doi.org/10.1128/JVI.77.2.1329-1336.2003
Department(s) Laboratory of Virology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) spotted wilt virus - nonstructural protein nss - s-rna segment - nicotiana-benthamiana - nucleotide-sequence - transgenic plants - expression - interference - determinants - bunyavirus
Abstract Posttranscriptional silencing of a green fluorescent protein (GFP) transgene in Nicotiana benthamiana plants was suppressed when these plants were infected with Tomato spotted wilt virus (TSWV), a plant-infecting member of the Bunyaviridae. Infection with TSWV resulted in complete reactivation of GFP expression, similar to the case for Potato virus Y, but distinct from that for Cucumber mosaic virus, two viruses known to carry genes encoding silencing suppressor proteins. Agrobacterium-based leaf injections with individual TSWV genes identified the NSS gene to be responsible for the RNA silencing-suppressing activity displayed by this virus. The absence of short interfering RNAs in NSS-expressing leaf sectors suggests that the tospoviral NSS protein interferes with the intrinsic RNA silencing present in plants. Suppression of RNA silencing was also observed when the NS3 protein of the Rice hoja blanca tenuivirus, A nonenveloped negative-strand virus, was expressed. These results indicate that plant-infecting negative-strand RNA viruses carry a gene for a suppressor of RNA silencing.
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