Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 326246
Title Endo-xylogalacturonan hydrolase
Author(s) Herweijer, M.A.; Vincken, J.P.; Meeuwsen, P.J.A.; Vlugt-Bergmans, C.J.B. van der; Beldman, G.; Ooyen, A.J.J. van; Voragen, A.G.J.
Source In: Advances in pectin and pectinase research / Voragen, A.G.J., Schols, H.A., Visser, R.G.F., Dordrecht : - ISBN 9781402011443 - p. 257 - 266.
Department(s) Food Chemistry
Publication type Chapter in scientific book
Publication year 2003
Keyword(s) pectinen - polygalacturonase - enzymen - appelsap - aspergillus - pectins - polygalacturonase - enzymes - apple juice - aspergillus
Categories Food Biotechnology
Abstract A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple
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