Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 326307
Title Conversion of 2-fluoromuconate to cis-dienelactone by purified enzymes from Rhodococcus opacus 1cp
Author(s) Solyanikova, I.P.; Moisseeva, O.V.; Boeren, S.; Boersma, M.G.; Kolomytseva, M.P.; Vervoort, J.J.M.; Rietjens, I.M.C.M.; Golovleva, L.A.; Berkel, W.J.H. van
Source Applied and Environmental Microbiology 69 (2003)9. - ISSN 0099-2240 - p. 5636 - 5642.
Department(s) Biochemistry
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) halogenated aromatic-compounds - alcaligenes-eutrophus jmp-134 - pseudomonas-azelaica hbp1 - modified ortho-pathway - phenol hydroxylase - chemical-structure - erythropolis 1cp - 2-hydroxybiphenyl 3-monooxygenase - chloromuconate cycloisomerases - chlorocatechol 1
Abstract The present study describes the 19F nuclear magnetic resonance analysis of the conversion of 3-halocatechols to lactones by purified chlorocatechol 1,2-dioxygenase (ClcA2), chloromuconate cycloisomerase (ClcB2), and chloromuconolactone dehalogenase (ClcF) from Rhodococcus opacus 1cp grown on 2-chlorophenol. The 3-halocatechol substrates were produced from the corresponding 2-halophenols by either phenol hydroxylase from Trichosporon cutaneum or 2-hydroxybiphenyl 3-mono-oxygenase from Pseudomonas azelaica. Several fluoromuconates resulting from intradiol ring cleavage by ClcA2 were identified. ClcB2 converted 2-fluoromuconate to 5-fluoromuconolactone and 2-chloro-4-fluoromuconate to 2-chloro-4-fluoromuconolactone. Especially the cycloisomerization of 2-fluoromuconate is a new observation. ClcF catalyzed the dehalogenation of 5-fluoromuconolactone to cis-dienelactone. The ClcB2 and ClcF-mediated reactions are in line with the recent finding of a second cluster of chlorocatechol catabolic genes in R. opacus 1cp which provides a new route for the microbial dehalogenation of 3-chlorocatechol
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