Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 326427
Title Extraction of carotenoids from feces enabling the bioavailability of ß-carotene to be studied in Indonesian children
Author(s) Lieshout, M. van; West, C.E.; Bovenkamp, P. van de; Wang, Y.; Sun, Y.; Breemen, R.B. van; Permaesih, D.; Muhilal, ; Verhoeven, M.A.; Creemers, A.F.L.; Lugtenburg, J.
Source Journal of Agricultural and Food Chemistry 51 (2003)17. - ISSN 0021-8561 - p. 5123 - 5130.
Department(s) Global Nutrition
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) mass-spectrometry - retinol - humans - bioefficacy
Abstract Previously, we have presented a method for quantifying beta-carotene bioavailability based on analysis in serum, following administration of C-13-labeled beta-carotene. Because stool samples can be collected noninvasively, we have now extended the method to measure the bioavailability based on measurements in feces. An extraction method was developed to enable measurement of concentrations and degree of isotopic enrichment of retinol, retinyl palmitate and carotenoids in feces. Relative bioavailability of beta-carotene from pumpkin (n = 6) was found to be 1.8 times (interval, 0.6, 5.5) greater than that from spinach (n = 8), based on data from feces compared with 1.7 times (interval, 0.9, 3.1) based on data from serum.
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