Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 326928
Title Rheology and structure of ovalbumin gels at low pH and low ionic strength
Author(s) Weijers, M.; Sagis, L.M.C.; Veerman, C.; Sperber, B.L.H.M.; Linden, E. van der
Source Food Hydrocolloids 16 (2002). - ISSN 0268-005X - p. 269 - 276.
Department(s) Physical Chemistry and Colloid Science
Physics and Physical Chemistry of Foods
Food Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2002
Keyword(s) hen ovalbumin - turbidity
Abstract The objective of this study was to relate the rheological behavior of ovalbumin gels at low pH and low ionic strength to their mesoscopic structure, using rheological measurements and Transmission Electron Microscopy (TEM). Varying pH, ionic strength and protein concentration, we obtained transparent solutions, transparent gels, opaque gels, or turbid gels, upon heating and subsequent cooling of the ovalbumin solutions. At equal pH and increasing salt concentration we found an increase in turbidity, suggesting that the structure of the aggregates changed from linear or branched to more clustered aggregates. The gel strength increased with increasing salt concentration. A similar trend was observed at equal ionic strength and increasing pH. TEM micrographs of ovalbumin aggregates at pH 2 and 15 mM NaCl show that the ovalbumin aggregates are linear. Increasing the pH from 2 to 3.5 results in structures that are still linear, but with a higher degree of clustering. No random aggregates were observed. TEM micrographs of the gel phase at pH 3.5 and 30 mM NaCl (turbid) show that the structure consists of linear aggregates organized in large clusters of approximately 230–350 nm. At pH 2 and 30 mM NaCl (transparent) the gel consists of single strands with a diameter of about 3.3–3.9 nm, i.e. one or at most two monomers thick.
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