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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 326979
Title Development of bacterial and bifidobacterial communities in feces of newborn babies
Author(s) Favier, C.F.; Vos, W.M. de; Akkermans, A.D.L.
Source Anaerobe 9 (2003). - ISSN 1075-9964 - p. 219 - 229.
DOI https://doi.org/10.1016/j.anaerobe.2003.07.001
Department(s) Microbiology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) 16s ribosomal-rna - gradient gel-electrophoresis - bottle-fed infants - human intestinal microflora - species-specific primers - formula-fed infants - fecal flora - microbial ecology - targeted probes - human-colon
Abstract Microbial 16S rDNA from babies' fecal samples were amplified by PCR, and analysed by denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. PCR-DGGE profiles were used to follow in time the colonization of the intestine by bacteria. Four healthy babies, one baby who received antibiotics and their parents participated to the present study to determine the extent to which administration of antibiotics can modify the bacterial colonization of neonatal human gut and verify the influence of parental factors on the formation of the fecal bacterial community. In the healthy babies, Escherichia coli or bacteria belonging to Clostridium spp. were the initial colonizers rapidly followed by Bifidobacterium, Bacteroides, Clostridium, Streptococcus, Enterococcus and Actinomyces. Bifidobacterium species appeared already after five days in the breast-fed babies while there was a delay in the baby who received a formula based diet during only one day after birth. In each baby two or three bifidobacterial species including B. infantis were found. The observed variations in species were not associated with the feeding changes. The comparison of DGGE profiles of the babies and their parents patterns showed bands with equal migration suggesting a vertical transmission determined by genetic and environmental factors. The brief appearance of pioneer bacteria determined as being E coli and Enterococcus spp. in the profile from the. baby under antibiotic therapy, was succeeded by a small stable community consisting of Ruminococcus species. No Bifidobacterium sequences were detectable in this antibiotic-treated baby in spite of a partly breast-milk diet. (C) 2003 Elsevier Ltd. All rights reserved.
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