Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327005
Title Molecular approaches for the detection and identification of bifidobacteria and lactobacilli in the human gastrointestinal tract
Author(s) Satokari, R.M.; Vaughan, E.E.; Smidt, H.; Saarela, M.; Matto, J.; Vos, W.M. de
Source Systematic and Applied Microbiology 26 (2003). - ISSN 0723-2020 - p. 572 - 584.
Department(s) Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) 16s ribosomal-rna - gradient gel-electrophoresis - lactic-acid bacteria - polymerase-chain-reaction - targeted oligonucleotide probes - species-specific primers - in-situ hybridization - human fecal samples - human intestinal microflora - dna restriction analysis
Abstract In this review an overview of various molecular techniques and their application for the detection and identification of bifidobacteria and lactobacilli in the gastrointestinal (GI) tract is presented. The techniques include molecular typing techniques such as amplified ribosomal DNA restriction analysis (ARDRA), randomly amplified polymorphic DNA (RAPD), pulsed field gel electrophoresis (PFGE), ribotyping and community profiling techniques such as PCR coupled to temperature and denaturing gradient gel electrophoresis (PCR-TGGE and PCR-DGGE, respectively). Special attention is given to oligonucleotide probes and primers that target the ribosomal RNA (rRNA) sequences and their use in PCR and different hybridisation techniques such as DNA microarrays and fluorescent in situ hybridisation (FISH). In addition, recent findings based on the molecular studies of bifidobacteria and lactobacilli in the GI-tract are reviewed.
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