Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327091
Title Evidence of medium-chain-length polyhydroxyoctanoate accumulation in transgenic potato lines expressing the Pseudomonas oleovorans Pha-C1 polymerase in the cytoplasm
Author(s) Romano, A.; Vreugdenhil, D.; Jamar, D.C.L.; Plas, L.H.W. van der; Roo, G. de; Witholt, B.; Eggink, G.; Mooibroek, A.
Source Biochemical Engineering Journal 16 (2003). - ISSN 1369-703X - p. 135 - 143.
Department(s) Laboratory of Plant Physiology
Agrotechnological Research Institute
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) recombinant escherichia-coli - beta-oxidation - fluorescent pseudomonads - molecular-basis - brassica-napus - plants - gene - polyhydroxyalkanoates - poly(3-hydroxyalkanoates) - aeruginosa
Abstract The phaC1 gene from Pseudomonas oleovorans, coding for the Pha-C1 polymerase, was introduced into the potato genome. Transgenic callus and plant lines which transcribed and translated the transgene were selected and cell suspension cultures from the wild type and transgenic lines were established. The substrate for the Pha-C1 polymerase, 3-(R)-hydroxyoctanoate, was provided to the growth medium. In the transgenic lines, but not in the wild type or in transgenic cell suspension cultures without Pha-C1 expression, evidence of medium-chain-length polyhydroxyalkanoate accumulation ranging from 0.02 to 9.7 mg of polymer per gram of dry weight was observed after feeding in the growth medium the substrate. (C) 2003 Elsevier Science B.V. All rights reserved.
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