Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327549
Title Degradation of 3,4-dichloro- and 3,4-difluoroaniline by Pseudomonas fluorescens 26K
Author(s) Travkin, V.; Solyanikova, I.P.; Rietjens, I.M.C.M.; Vervoort, J.J.M.; Berkel, W.J.H. van; Golovleva, L.A.
Source Journal of Environmental Science and Health. Part B, Pesticides Food Contaminants, and agricultural wastes 38 (2003). - ISSN 0360-1234 - p. 121 - 132.
DOI https://doi.org/10.1081/PFC-120018443
Department(s) Sub-department of Toxicology
Biochemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) microbial-degradation - aniline - chloroanilines - soil
Abstract 3,4-Dichloro- and 3,4-difluoroanilines were degraded by Pseudomonas fluorescens 26-K under aerobic conditions. In the presence of glucose strain degraded 170 mg/L of 3,4-dichloroaniline (3,4-DCA) during 2-3 days. Increasing of toxicant concentration up to 250 mg/L led to degradation of 3,4-DCA during 4 days and its intermediates during 5-7 days. Without cosubstrate and nitrogen source degradation of 3,4-DCA took place too, but more slowly-about 40% of toxicant at initial concentration 75 mg/L was degraded during 15 days. 3,4-Difluoroaniline (3,4-DFA) (initial concentration 170 mg/L) was degraded by Pseudontonas fluorescens 26-K during 5-7 days. The strain was able to completely degrade up to 90 mg/L of 3,4-DFA, without addition of cosubstrate and nitrogen during 15 days. Degradation of fluorinated aniline was accompanied by intensive defluorination. Activity of catechol 2,3-dioxygenase (C2,3DO) (0.230 mumol/min/mg of protein) was found in the culture liquid of the strain, grown with 3,4-DCA and glucose. This fact, as well as, the presence of 3-chloro-4-hydroxyaniline as a metabolite suggested that 3,4-DCA degradation pathway includes dehalogenation and hydroxylation of aromatic ring followed by its subsequent cleaving by C2,3DO. On the contrary, activity of catechol 1,2-dioxygenase (C1,2DO) (0.08 mumol/min/mg of protein) was found in the cell-free extract of biomass grown on 3,4-DFA. 3 -Fluoro-4-hydroxyani line as intermediate was found in this cell-free extract.
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