Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 332952
Title A novel method for the construction of genome wide transcriptome maps
Author(s) Brugmans, B.W.; Fernandez del Carmen, A.; Bachem, C.W.B.; Os, H. van; Eck, H.J. van; Visser, R.G.F.
Source The Plant Journal 31 (2002)2. - ISSN 0960-7412 - p. 211 - 222.
Department(s) Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2002
Keyword(s) differential gene-expression - cdna-aflp - linkage maps - arabidopsis-thaliana - potato - markers - rflp - sequence - tomato - profiles
Abstract Expression profiling by cDNA-AFLP is commonly used to display the transcriptome of a specific tissue, treatment or developmental stage. In this paper, cDNA-AFLP has been used to study transcripts expressed in segregating populations from Arabidopsis thaliana and potato (Solanum tuberosum ). The genetic differences between the offspring genotypes are thus visualized as polymorphisms in the transcriptome. We show that polymorphic transcripts can be used as genetic markers and allow the construction of a linkage map. The resulting map shows that, in contrast to genomic markers, the transcriptome-derived markers did not cluster in particular areas of the chromosome, and that cDNA-AFLP markers are targeted specifically to transcriptionally active regions. The cDNA-AFLP markers used in mapping are derived from DNA polymorphisms in transcripts, rather than differences in expression regulation. The high potential of transcriptome markers as opposed to (anonymous) genomic markers for applications in genetic analyses, marker-assisted breeding and map-based cloning is discussed.
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