Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 333104
Title The self-incompatibility response in Papaver rhoeas pollen causes early and striking alterations to organelles
Author(s) Geitmann, A.; Franklin-Tong, V.E.; Emons, A.M.C.
Source Cell Death and Differentiation 11 (2004)8. - ISSN 1350-9047 - p. 812 - 822.
DOI https://doi.org/10.1038/sj.cdd.4401424
Department(s) Laboratory of Cell Biology
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) programmed-cell-death - sunflower helianthus-annuus - induced apoptosis - hypersensitive response - mitochondrial structure - actin polymerization - free calcium - tube growth - hl-60 cells - protein
Abstract Self-incompatibility (SI) in Papaver rhoeas is accompanied by a cascade of signalling events that result in the rapid arrest and eventual death of the pollen tube. We have used rapid freeze fixation, freeze substitution and transmission electron microscopy to provide the first description of changes to pollen at the ultrastructural level during SI in this species. Our studies reveal that dramatic alterations to the morphology of mitochondria, Golgi bodies and ER occur within 1 h of SI induction. Similar symptoms have also been observed during programmed cell death (PCD) in some cell types. These include: the conspicuous condensation of the vegetative and generative nuclei, the swelling and loss of cristae in mitochondria and the disappearance of Golgi bodies. Some of the early alterations to the mitochondria and Golgi bodies observed at 1 h, almost certainly occur when cells are still alive. Other events, such as nuclear condensation, occur later and coincide with DNA fragmentation and the loss of cell viability. Our observations suggest that the SI response in P. rhoeas pollen may potentially involve a type of PCD.
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