Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 333185
Title The plasma membrane aquaporin NtAQP1 is a key component of the leaf unfolding mechanism in tobacco
Author(s) Siefritz, F.; Otto, B.; Beinert, G.P.; Krol, A.R. van der; Kaldenhoff, R.
Source The Plant Journal 37 (2004). - ISSN 0960-7412 - p. 147 - 155.
DOI https://doi.org/10.1046/j.1365-313X.2003.01947.x
Department(s) Laboratory of Plant Physiology
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) nicotiana-tabacum - gene-expression - regulated gene - water - promoter - expansion - growth - auxin - plants - phosphorylation
Abstract Epinastic leaf movement of tobacco is based on differential growth of the upper and lower leaf surface and is distinct from the motor organ-driven mechanism of nyctinastic leaf movement of, for example, mimosa species. The epinastic leaf movement of tobacco is observed not only under diurnal light regimes but also in continuous light, indicating a control by light and the circadian clock. As the transport of water across membranes by aquaporins is an important component of rapid plant cell elongation, the role of the tobacco aquaporin Nt aquaporin (AQP)1 in the epinastic response was studied in detail. In planta NtAQP1-luciferase (LUC) activity studies, Northern and Western blot analyses demonstrated a diurnal and circadian oscillation in the expression of this plasma membrane intrinsic protein (PIP)1-type aquaporin in leaf petioles, exhibiting peaks of expression coinciding with leaf unfolding. Cellular water permeability of protoplasts isolated from leaf petioles was found to be high in the morning, i.e. during the unfolding reaction, and low in the evening. Moreover, diurnal epinastic leaf movement was shown to be reduced in transgenic tobacco lines with an impaired expression of NtAQP1. It is concluded that the cyclic expression of PIP1-aquaporin represents an important component of the leaf movement mechanism.
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