Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 333962
Title Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium
Author(s) Derrien, M.M.N.; Vaughan, E.E.; Plugge, C.M.; Vos, W.M. de
Source International Journal of Systematic and Evolutionary Microbiology 54 (2004)5. - ISSN 1466-5026 - p. 1469 - 1476.
Department(s) Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) gradient gel-electrophoresis - 16s ribosomal-rna - butyrate-producing bacterium - human-colon ecosystems - human feces - sequence-analysis - in-vitro - reveals - growth - prosthecobacter
Abstract The diversity of mucin-degrading bacteria in the human intestine was investigated by combining culture and 16S rRNA-dependent approaches. A dominant bacterium, strain Muc(T), was isolated by dilution to extinction of faeces in anaerobic medium containing gastric mucin as the sole carbon and nitrogen source. A pure culture was obtained using the anaerobic soft agar technique. Strain Muc(T) was a Gram-negative, strictly anaerobic, non-motile, non-spore-forming, oval-shaped bacterium that could grow singly and in pairs. When grown on mucin medium, cells produced a capsule and were found to aggregate. Strain Muc(T) could grow on a limited number of sugars, including N-acetylglucosamine, N-acetylgalactosamine and glucose, but only when a protein source was provided and with a lower growth rate and final density than on mucin. The G+C content of DNA from strain Muc(T) was 47.6 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the division Verrucomicrobia. The closest described relative of strain Muc(T) was Verrucomicrobium spinosum (92% sequence similarity). Remarkably, the 16S rRNA gene sequence of strain Muc(T) showed 99% similarity to three uncultured colonic bacteria. According to the data obtained in this work, strain Muc(T) represents a novel bacterium belonging to a new genus in subdivision 1 of the Verrucomicrobia; the name Akkermansia muciniphila gen. nov., so. nov. is proposed; the type strain is Muc(T) ( = ATCC BAA-835(T) = CIP 107961(T)).
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