Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 333990
Title Histochemical analysis reveals organ-specifics Quantitative Trait Loci for enzyme activities in Arabidopsis
Author(s) Sergeeva, L.I.; Vonk, J.; Keurentjes, J.J.B.; Plas, L.H.W. van der; Koornneef, M.; Vreugdenhil, D.
Source Plant Physiology 134 (2004)1. - ISSN 0032-0889 - p. 237 - 245.
Department(s) Laboratory of Plant Physiology
Laboratory of Genetics
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) wild-type - starchless mutant - qtl analysis - thaliana - phosphoglucomutase - metabolism - deficient - growth - map
Abstract To identify genetic loci involved in the regulation of organ-specific enzyme activities, a specific histochemical staining protocol was used in combination with quantitative trait locus (QTL) analysis. Using phosphoglucomutase (PGM) as an example, it is shown that enzyme activity can specifically, and with high resolution, be visualized in non-sectioned seedlings of Arabidopsis. The intensities of staining were converted to quantitative data and used as trait for QTL analysis using Landsberg erecta x Cape Verde Islands recombinant inbred lines. Independently, PGM activities were quantified in whole-seedling extracts, and these data were also used for QTL analysis. On the basis of extract data, six significant (P <0.05) loci affecting PGM activity were found. From the histochemical data, one or more specific QTLs were found for each organ analyzed (cotyledons, shoot apex, hypocotyl, root, root neck, root tip, and root hairs). Loci detected for PGM activity in extracts colocated with loci for histochemical staining. QTLs were found coinciding with positions of (putative) PGM genes but also at other positions, the latter ones supposedly pointing toward regulatory genes. Some of this type of loci were also organ specific. It is concluded that QTL analysis based on histochemical data is feasible and may reveal organ-specific loci involved in the regulation of metabolic pathways.
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