Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 334208
Title Degradation of lucerne stem cell walls by five rumen bacterial species
Author(s) Jung, H.G.; Engels, F.M.; Weimer, P.J.
Source NJAS Wageningen Journal of Life Sciences 52 (2004)1. - ISSN 1573-5214 - p. 11 - 28.
DOI https://doi.org/10.1016/S1573-5214(04)80027-7
Department(s) Department of Plant Sciences
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) ruminal bacteria - pure cultures - cellulolytic bacteria - hemicellulose - digestibility - digestion - tissues - extent - degradability - forages
Abstract The rumen bacterial strains Butyrivibrio fibrisolvens H17c, Fibrobacter succinogenes S85, Lachnospira multiparus 40, Ruminococcus albus 7 and R. flavefaciens FD-1 were compared individually and as a five-species mixture with a rumen inoculum for their ability to degrade lucerne (Medicago sativa L.) stem cell wall polysaccharides. Two maturity stages of lucerne (bud and full flower) were utilized as substrates and incubation periods of 24 and 96 h were employed. Lucerne stem substrates and fermentation residues were analysed for cell wall content and composition. Degradation of total cell wall polysaccharides and all polysaccharide fractions was greater for immature than for mature lucerne stems. The 96-h incubation resulted in significantly more degradation of all cell wall polysaccharides than did the 24-h incubation. The R. albus culture was able to degrade all cell wall polysaccharide fractions as well or better than any other culture, including the rumen inoculum. Co-culture of R. albus with the four other ruminal species did not improve degradation of any wall polysaccharides compared with R. albus alone. Microscopic examination indicated that R. albus and the five-species mixture appeared to degrade Lucerne tissues in thin sections to almost the same extent as did rumen fluid.
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