Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 335036
Title Design of a confocal microfluidic particle sorter using fluorescent photon burst detection
Author(s) Kunst, B.H.; Schots, A.; Visser, A.J.W.G.
Source Review of Scientific Instruments 75 (2004)9. - ISSN 0034-6748 - p. 2892 - 2898.
Department(s) Biochemistry
Laboratory of Nematology
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) single-molecule detection - correlation spectroscopy - submicrometer channels - cross-correlation - fluidic channels - flow-cytometry - cell sorter - dna - microcapillary - confinement
Abstract An instrumental system is described for detecting and sorting single fluorescent particles such as microspheres, bacteria, viruses, or even smaller macromolecules in a flowing liquid. The system consists of microfluidic chips (biochips), computer controlled high voltage power supplies, and a fluorescence microscope with confocal optics. The confocal observation volume and detection electro-optics allow measurements of single flowing fluorescent particles. The output of the avalanche photodiode (single photon detector) is coupled to a real-time photon-burst detection device, which output can address the control of high voltage power supplies for sorting purposes. Liquid propulsion systems like electro-osmotic flow and plain electric fields to direct the particles through the observation volume have been tested and evaluated. The detection and real-time sorting of fluorescent microspheres are demonstrated. Applications of these biochips for screening of bacteriophages-type biolibraries are briefly discussed. (C) 2004 American Institute of Physics.
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