Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 335044
Title Cell cycle activity and beta-tubulin accumulation during dormancy breaking of Acer platanoides L. seeds
Author(s) Pawlowski, T.A.; Bergervoet, J.H.W.; Bino, R.J.; Groot, S.P.C.
Source Biologia Plantarum 48 (2004)2. - ISSN 0006-3134 - p. 211 - 218.
DOI https://doi.org/10.1023/B:BIOP.0000033447.27149.85
Department(s) PRI Bioscience
Laboratory of Plant Physiology
EPS-3
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) flow cytometric determination - nuclear replication activity - fir pseudotsuga-menziesii - brassica-oleracea l. - norway maple - warm stratification - qualitative changes - protein-synthesis - early germination - plant development
Abstract Cell cycle events in embryo axes of Norway maple (Acer platanoides L.) seeds were studied during dormancy breaking by flow cytometric analyses of the nuclear DNA content and by immunodetection of beta-tubulin. Most embryonic nuclei of dry, fully matured seeds were arrested in the G(2) phase of the cell cycle. In addition, the lowest content of beta-tubulin was detected in dry, mature seeds. Imbibition in water and cold stratification resulted in a decrease in the number of nuclei in G(2), and a simultaneous increase in beta-tubulin content. In germinated seeds the content of beta-tubulin was the highest and the number of cells in G(2) was the lowest. Both cell cycle events preceded cell expansion and division and subsequent growth of the radicle through the seed coat. The anatomical investigation has proved that the main reason for decrease in the number of nuclei in G(2) is mitosis, started with seeds germination ( radicle protrusion). The activation of the cell cycle and the beta-tubulin accumulation were associated with embryo dormancy breaking.
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