Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 335272
Title Towards sorting of biolibraries using single-molecule fluorescence detection techniques
Author(s) Visser, A.J.W.G.; Kunst, B.H.; Keller, H.J.H.G.; Schots, A.
Source Current Pharmaceutical Biotechnology 5 (2004). - ISSN 1389-2010 - p. 173 - 179.
DOI https://doi.org/10.2174/1389201043376977
Department(s) Biochemistry
Laboratory of Nematology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) cross-correlation spectroscopy - total analysis systems - phage-display - cell sorter - flow - biotechnology - technology - particles - channels - cloning
Abstract The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation or biolibraries, single-molecule detection techniques and rnicrofluidic devices. A high-throughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.
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