Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 335425
Title A conserved transcript pattern in response to a specialist and a generalist herbivore
Author(s) Reymond, Ph.; Bodenhausen, N.; Poecke, R.M.P. van; Krishnamurthy, V.; Dicke, M.; Farmer, E.E.
Source The Plant Cell 16 (2004)11. - ISSN 1040-4651 - p. 3132 - 3147.
DOI https://doi.org/10.1105/tpc.104.026120
Department(s) Laboratory of Entomology
EPS-2
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2004
Keyword(s) manduca-sexta lepidoptera - host nicotiana-attenuata - plant defense responses - egyptian cotton worm - large-scale changes - jasmonic acid - molecular-interactions - arabidopsis-thaliana - signaling pathways - microarray analysis
Abstract Transcript patterns elicited in response to attack reveal, at the molecular level, how plants respond to aggressors. These patterns are fashioned both by inflicted physical damage as well as by biological components displayed or released by the attacker. Different types of attacking organisms might therefore be expected to elicit different transcription programs in the host. Using a large-scale DNA microarray, we characterized gene expression in damaged as well as in distal Arabidopsis thaliana leaves in response to the specialist insect, Pieris rapae. More than 100 insect-responsive genes potentially involved in defense were identified, including genes involved in pathogenesis, indole glucosinolate metabolism, detoxification and cell survival, and signal transduction. Of these 114 genes, 111 were induced in Pieris feeding, and only three were repressed. Expression patterns in distal leaves were markedly similar to those of local leaves. Analysis of wild-type and jasmonate mutant plants, coupled with jasmonate treatment, showed that between 67 and 84% of Pieris-regulated gene expression was controlled, totally or in part, by the jasmonate pathway. This was correlated with increased larval performance on the coronatine insensitive1 glabrous1 (coi1-1 gl1) mutant. Independent mutations in COI1 and GL1 led to a faster larval weight gain, but the gl1 mutation had relatively little effect on the expression of the insect-responsive genes examined. Finally, we compared transcript patterns in Arabidopis in response to larvae of the specialist P. rapae and to a generalist insect, Spodoptera littoralis. Surprisingly, given the complex nature of insect salivary components and reported differences between species, almost identical transcript profiles were observed. This study also provides a robustly characterized gene set for the further investigation of plant-insect interaction.
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