Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 336941
Title Identification of intermediates and enzymes involved in the early steps of artemisinin biosynthesis in Artemisia annua
Author(s) Bertea, C.M.; Freije, J.R.; Woude, H. van der; Verstappen, F.W.A.; Perk, L.; Marquez, V.; Kraker, J.W. de; Posthumus, M.A.; Jansen, B.J.M.; Groot, Æ. de; Franssen, M.C.R.; Bouwmeester, H.J.
Source Planta Medica 71 (2005)1. - ISSN 0032-0943 - p. 40 - 47.
DOI https://doi.org/10.1055/s-2005-837749
Department(s) Toxicology
PRI Bioscience
Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) dihydroartemisinic acid - amorpha-4,11-diene synthase - chicory - roots
Abstract An important group of antimalarial drugs consists of the endoperoxide sesquiterpene lactone artemisinin and its derivatives. Only little is known about the biosynthesis of artemisinin in Artemisia annua L., particularly about the early enzymatic steps between amorpha-4,11-diene and dihydroartemisinic acid. Analyses of the terpenoids from A. annua leaves and gland secretory cells revealed the presence of the oxygenated amorpha-4,11-diene derivatives artemisinic alcohol, dihydroartemisinic alcohol, artemisinic aldehyde, dihydroartemisinic aldehyde and dihydroartemisinic acid. We also demonstrated the presence of a number of biosynthetic enzymes such as the amorpha-4,11-diene synthase and the - so far unknown - amorpha-4,11-diene hydroxylase as well as artemisinic alcohol and dihydroartemisinic aldehyde dehydrogenase activities in both leaves and glandular trichomes. From these results, we hypothesise that the early steps in artemisinin biosynthesis involve amorpha-4,11-diene hydroxylation to artemisinic alcohol, followed by oxidation to artemisinic aldehyde, reduction of the C11-C13 double bond to dihydroartemisinic aldehyde and oxidation to dihydroartemisinic acid
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