Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 339306
Title Characteristics of endogenous flavin fluorescence of Photobacterium leiognathi luciferase and Vibrio fisheri NAD(P)H:FMN-oxidoreductase
Author(s) Vetrova, E.V.; Kudryasheva, N.S.; Visser, A.J.W.G.; Hoek, A. van
Source Luminescence 20 (2005)3. - ISSN 1522-7235 - p. 205 - 209.
DOI https://doi.org/10.1002/bio.815
Department(s) Biochemistry
Biophysics
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) flavodoxin - anisotropy - reductase - dynamics - system
Abstract The bioluminescent bacterial enzyme system NAD(P)H:FMN-oxidoreductase-luciferase has been used as a test system for ecological monitoring. One of the modes to quench bioluminescence is the interaction of xenobiotics with the enzymes, which inhibit their activity. The use of endogenous flavin fluorescence for investigation of the interactions of non-fluorescent compounds with the bacterial luciferase from Photobacterium leiognathi and NAD(P)H:FMN-oxidoreductase from Vibrio fischeri has been proposed. Fluorescence spectroscopy methods have been used to study characteristics of endogenous flavin fluorescence (fluorophore lifetime, the rotational correlation time). The fluorescence anisotropy behaviour of FMN has been analysed and compared to that of the enzyme-bound flavin. The fluorescence characteristics of endogenous flavin of luciferase and NAD(P)H:FMN-oxidoreductase have been shown to be applicable in studying enzymes' interactions with non-fluorescent compounds
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