Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 34282
Title Molecular basis of gene-specific RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants.
Author(s) Prins, M.; Haan, P. de; Goldbach, R.
Source Acta Horticulturae 431 (1996). - ISSN 0567-7572 - p. 449 - 458.
Department(s) Laboratory of Virology
Publication type Non-refereed article in scientific journal
Publication year 1996
Abstract Transgenic resistance to tomato spot wilt virus (TSWV) has been accomplished by expressing the viral nucleocapsid (N) protein gene in transgenic tobacco. Similar levels of protection, i.e., complete immunity to the virus in homozygous S2 plants, have been observed when an untranslatable N protein gene was expressed, indicating that the N protein gene-based resistance is mediated by the expressed transgene viral RNA. To test whether this RNA-mediated resistance is limited to plants that express viral N protein RNA sequences, 325 transgenic plant lines were produced that expressed a wide range of parts of the TSWV RNA-genome or its complement, spanning over 70␘f the TSWV genome, including the entire S and M RNA sequences. The progenies of these plants were screened for resistance to TSWV. Remarkably, only plants that expressed N or NSm gene sequences displayed immunity to the virus. The functions of these proteins may be blocked early in the infection process, suggesting an important role for these proteins in early stages of systemic virus infection. The mechanism by which the expression of transgenic RNAs can cause immunity in transformed plants is discussed.
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