Rumen escape protein (REP) was determined for six grasses and 16 grass silages using a nylon bag technique and an in vitro technique using a proteolytic enzyme preparation of Streptomyces griseus. In vitro, the samples were incubated for 0, 1, 6 and 24 h. The highest correlation observed between percentage REP (%REP), determined with the nylon bag technique, and the in vitro undegradable fraction (U) occurred at 24 h of incubation (%U24; R2=0.71). Shorter incubation periods weakened the relationship (R2=0.46¿0.59). Percentage REP could be estimated from %U24 as %REP=3.3 (±4.02) + 1.31 (±0.19) *%U24. For the incubation periods investigated, results show that the highest relationship with %REP occurred with an incubation period of 24 h for these grass and grass silage samples.
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