Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 345804
Title Isoprenoid biosynthesis in Artemisia annua: Cloning and heterologous expression of a germacrene A synthase from a glandular trichome cDNA library
Author(s) Bertea, C.M.; Voster, A.; Verstappen, F.W.A.; Maffei, M.; Beekwilder, M.J.; Bouwmeester, H.J.
Source Archives of Biochemistry and Biophysics 448 (2006)1-2. - ISSN 0003-9861 - p. 3 - 12.
DOI https://doi.org/10.1016/j.abb.2006.02.026
Department(s) PRI Bioscience
Laboratory of Plant Physiology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) amorpha-4,11-diene synthase - bacterial expression - arabidopsis-thaliana - diphosphate synthase - molecular-cloning - key enzyme - l. - (e)-beta-farnesene - sesquiterpenes - accumulation
Abstract Artemisia annua (Asteraceae) is the source of the anti-malarial compound artemisinin. To elucidate the biosynthetic pathway and to isolate and characterize genes involved in the biosynthesis of terpenoids including artemisinin in A. annua, glandular trichomes were used as an enriched source for biochemical and molecular biological studies. The sequencing of 900 randomly selected clones from a glandular trichome plasmid cDNA library revealed the presence of many ESTs involved in isoprenoid biosynthesis such as enzymes from the methylerythritol phosphate pathway and the mevalonate pathway, amorpha-4,11-diene synthase and other sesquiterpene synthases, monoterpene synthases and two cDNAs showing high similarity to germacrene A synthases. Full-length sequencing of the latter two ESTs resulted in a 1686-bp ORF encoding a protein of 562aa. Upon expression in Escherichia coli, the recombinant protein was inactive with geranyl diphosphate, but catalyzed the cyclization of farnesyl diphosphate to germacrene A. These results demonstrate the potential of the use of A. annua glandular trichomes as a starting material for studying isoprenoid biosynthesis in this plant species.
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